Olympus BX-POL Instruction Manual
![Olympus BX-POL manual cover](/images/f89e63a27be4ed1653e168da/thumbs/thumb175x175.webp)
To align the polarizer, first ensure that the analyzer is removed or rotated out of the light path. Rotate the polarizer until the background light is minimized and the field of view appears darkest, indicating extinction is achieved.
Use a blower to remove dust, then gently wipe the lenses with a lens cleaning paper or cloth moistened with a small amount of lens cleaning solution. Avoid excessive pressure to prevent scratches.
Adjust the condenser by centering it with the field diaphragm and then raising or lowering it until the light is evenly distributed across the field of view. Close the diaphragm to enhance contrast if necessary.
Check that the objective lens is correctly clicked into position, ensure the sample is properly mounted, clean the lenses, and adjust the focus knobs carefully. Also verify that the condenser and diaphragm settings are correct for your sample.
Center and focus the field diaphragm, then adjust the condenser height to focus the edges of the diaphragm. Open the diaphragm until it just disappears from the field of view and ensure the light path is aligned.
Regularly clean the lenses and stage, check for dust on optical components, ensure mechanical parts are lubricated if needed, and periodically verify alignment of optical components. Store the microscope with a dust cover.
Ensure all optical components are properly aligned, the polarizer and analyzer are set correctly, and the Köhler illumination is properly adjusted. Consistent sample preparation is also crucial.
Check for debris or obstructions on the stage rails, clean them if necessary, and apply a small amount of appropriate lubricant. Ensure that the tension adjustment is not overly tight.
The Olympus BX-POL is primarily designed for polarized light microscopy. However, with appropriate modifications and filters, it may be adapted for basic fluorescence applications. Consult the manufacturer for specific upgrade options.
Place a stage micrometer on the stage, focus on the scale, and align it with the ocular micrometer. Calculate the calibration factor by dividing the known distance on the stage micrometer by the number of divisions on the ocular micrometer.